Coding
h

Part:BBa_K4899006:Design

Designed by: Morten Pedersen Hauger   Group: iGEM23_UiOslo-Norway   (2023-10-11)


His-tagged dead cas12f, CasMINI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1417
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1417
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1417
    Illegal BglII site found at 350
    Illegal BglII site found at 359
    Illegal BglII site found at 428
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1417
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1417
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

modeling to ensure tag does not interfere with binding and dimer formation


Source

literture, and own design

References